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ATP citrate lyase antibody [N1

N2], N-term
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月13日
  • WB, ICC/IF, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX112387

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P

    • 浓度

      0.55 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      ATP citrate lyase

    • 抗体英文名

      ATP citrate lyase antibody [N1N2], N-term

    • 抗体名

      ATP citrate lyase 抗体 [N1N2], N-term

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
    Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
    Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
    Blue: Hoechst 33342 staining.

    产品细节图片2

    Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片3

    Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase(GTX112387) antibody at 1:500 dilution.
    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片4

    ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse pancreas.
    ATP citrate lyase stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片5

    Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Li X et al., Mol Cell 2017 (PMID:28552616)
    Garc?a-Arroyo FE et al., Biomolecules 2019 (PMID:31614639)
    相关实验
    • 裂解酶 lyase

      为酶分类的一个主要类群,可催化基质脱除基团,从一个化合物形成两个化合物的酶之总称(酶编号1位数字为 4)。也可说是能使一个分子在双键上结合一个另外的分子,而形成一个新的分子的酶。根据解裂的键的种类( C- C、 C- O、 C- N等)再分成更细的类。有一些参与重要代谢反应的酶,例如参与 C- C键裂解和形成的醛缩酶(脱醛酶之一)、脱羧酶(产生 CO2 )、柠檬酸合成酶(与 CoA的水解相伴导向合成方向)等;与 C- O键裂解有关的延胡索酸水合酶、烯醇化酶以及参与 C- N键裂解的天门

    • Monoclonal Antibody Production Protocol

      the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series

    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    ATP citrate lyase antibody [N1N2], N-term
    ¥1700 - 4000