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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human RAB3C Recombinant protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX108610
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RAB3C
- 抗体英文名:
RAB3C antibody [N1C3]
- 抗体名:
RAB3C 抗体 [N1C3]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB3C antibody [N1C3] (GTX108610) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RAB3C antibody [N1C3] detects RAB3C protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% PFA at RT for 15 min.
Green: RAB3C stained by RAB3C antibody [N1C3] (GTX108610) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX108610 diluted at 1:1000
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108610 diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using RAB3C(GTX108610) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Chang YC et al., Mol Cancer 2017 (PMID:28784136)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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