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Neobioscience
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文献和实验When trying to identify a clone within a cDNA library, which may contain a coat protein (CP) gene, one useful technique may by immunological screening, using antibodies raised against either purified virus or isolated CP. Antibody screening
: aliquot a portion of the stock to a separate tube and dilute to OD600 = 0.5-0.7 using 10mM MgSO4 PROCEDURE - Step 2 - Determine Library titer and Library plating 1. Grow ON culture of Y
Yeast Two-Hybrid Library Screening
The two-hybrid system was originally devised by Fields and Song as a protein interaction detection system in yeast (1 ). Subsequently, it has been employed in many laboratories as a means of screening cDNA and genomic fusion libraries
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