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Neobioscience
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文献和实验Methyltransferase-based single-promoter analysis assay
of nuclei with the CpG-specific DNA methyltransferase SssI (M.SssI), followed by genomic bisulfite sequencing of individual progeny DNA molecules (Fig. 1) (11-13) (see comment 1). This gives single molecule resolution over the promoter and allows
correlation between G:C→A:T transitions in the TP53 gene and promoter methylation of the O6 -methylguanine-DNA methyltransferase (MGMT) gene in glio-mas have been reported in several studies, suggesting the possible involvement of O 6 -methylguanine DNA
range of protein-DNA interactions by DNA methylation protection rather than nuclease-based strand scission (Jessen et al., 2006). Independently, a similar approach was developed, referred to as methyltransferase-based single-promoter analysis (MSPA
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