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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Aurora Kinase C (AURKC)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 32.7kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Gly92~Pro339 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
GNVYLAREK KSRFIVALKI LFKSQIEKEG VEHQLRREIE IQAHLKHPNI LQLYNYFYDQ QRIYLILEYA PRGELYKELQ KSGTFDEQRT ATIMEELSDA LMYCHKKKVI HRDIKPENLL LGLQGELKIA DFGWSVHAPS LRRKTMCGTL DYLPPEMIEG RMHNEMVDLW CIGVLCYELM VGNPPFESPS HSETYRRIVK VDLKFPSSMP LGAKDLISKL LKHNPSQRLP LEQVSAHPWV RANSRRVLP
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
We previously isolated Aurora-C (Aurkc/Aie1 ) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment
， Stuttgart, Germany) 。 ( 9 ) 成像板（BAS-SR 2025，Fujifilm，Japan) 。 ( 10 ) 磷屏显像仪（BAS-Reader-5000，Fujifilm) 。4. 注释 ( 1 ) 我们建议在激酶中加入甘油 [ 终浓度为 20% ( V/V）] 后保存在 -80°C，或将激酶在合适的缓冲液环境中冷冻干燥后保存在 -80°C，使用前，冻干的激酶要先用 ddH2O 溶解。 ( 2 ) 在进行基于芯片的激酶实验前，我们建议对激酶制备物进行胶内活性
，PD-1/PD-L1这么值钱，大家一定好奇它到底是什么鬼。 PD-1全名是程序性死亡分子1(programmed death-1)是Ishida等[1]通过消减杂交技术于1992年发现的[2]，是CD28家族成员，胞质区含有2个酪氨酸残基，1个位于靠近N端的免疫受体酪氨酸抑制基序(ITIM)中，另一个1个位于靠近C端的免疫受体酪氨酸转化基序(ITSM)中[2]。 PD-L1(CD274)是B7家族成员，是PD-1的配体，属于1型跨膜蛋白，全长290个氨基酸，包含1个IgV样
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