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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4)
- 库存:
1000
- 供应商:
上海信裕
泛素羧基端酯酶L4(UCHL4)重组蛋白
Recombinant Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 30.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu2~Ala233 with two N-terminal Tags, His-tag and T7-tag |
泛素羧基端酯酶L4(UCHL4)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
EGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMESE LLSIIPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGT IGLIHAIANN KDKVHFESGS TLKKFLEESV SMSPEERAKY LENYDAIRVT HETSAHEGQT EAPSIDEKVD LHFIALVHVD GHLYELDGWK PFPINHGKTS DETLLEDVIK VCKKFMERDP DELRFNAIAL SAA
EGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMESE LLSIIPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGT IGLIHAIANN KDKVHFESGS TLKKFLEESV SMSPEERAKY LENYDAIRVT HETSAHEGQT EAPSIDEKVD LHFIALVHVD GHLYELDGWK PFPINHGKTS DETLLEDVIK VCKKFMERDP DELRFNAIAL SAA
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
泛素羧基端酯酶L4(UCHL4)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
泛素羧基端酯酶L4(UCHL4)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
了一种诱导巨噬细胞焦亡的结核蛋白 EST12。EST12 结合巨噬细胞中活化的 C 激酶 1(RACK1)的受体,EST12-RACK1 复合体募集去泛素化酶 UCHL5 来促进 NLRP3 的 K48 连接去泛素化,随后导致 NLRP3–caspase-1/11–GSDMD–IL-1β免疫过程。EST12 晶体结构分析表明,氨基酸 Y80 是 RACK1 的关键结合位点。EST12 敲除菌株在体内或体外 MTB 感染中都显示出了高易感性。该研究首次证明了 RACK1 作为病原体的内源宿主感应蛋白
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泛素羧基端酯酶L4(UCHL4)重组蛋白
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