相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Glutaredoxin 3 (GLRX3)
- 库存:
1000
- 供应商:
上海信裕
谷氧还蛋白3(GLRX3)重组蛋白
Recombinant Glutaredoxin 3 (GLRX3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 31.7kDa |
| Concentration | 6.4 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu73~Val320 (Accession # Q9CQM9) with two N-terminal Tags, His-tag and T7-tag |
谷氧还蛋白3(GLRX3)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EAVPEVSE KYEISSVPTF LFFKNSQKVD RLDGAHAPEL TKKVQRHVSS GAFPPSTNEH LKEDLSLRLK KLTHAAPCML FMKGTPQEPR CGFSKQMVEI LHKHNIQFSS FDIFSDEEVR QGLKTYSNWP TYPQLYVSGE LIGGLDIIKE LEASEELDTI CPKAPKLEER LKVLTNKASV MLFMKGNKQE AKCGFSKQIL EILNSTGVEY ETFDILEDEE VRQGLKTFSN WPTYPQLYVR GDLVGGLDIV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EAVPEVSE KYEISSVPTF LFFKNSQKVD RLDGAHAPEL TKKVQRHVSS GAFPPSTNEH LKEDLSLRLK KLTHAAPCML FMKGTPQEPR CGFSKQMVEI LHKHNIQFSS FDIFSDEEVR QGLKTYSNWP TYPQLYVSGE LIGGLDIIKE LEASEELDTI CPKAPKLEER LKVLTNKASV MLFMKGNKQE AKCGFSKQIL EILNSTGVEY ETFDILEDEE VRQGLKTFSN WPTYPQLYVR GDLVGGLDIV
USAGE
谷氧还蛋白3(GLRX3)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
谷氧还蛋白3(GLRX3)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
键的还原。即硫氧还蛋白系统,该系统由硫氧还蛋白还原酶和硫氧还蛋白组成;谷氧还蛋白系统,该系统由谷胱甘肽还原酶、谷胱甘肽和三种谷氧还蛋白组成[143]。制造次还原细胞质环境,以利于二硫键形成的策略包括选用硫氧还蛋白还原酶(trxB)缺陷的E.coli菌株,这有助于巯基还原势能。 蛋白质在细胞质中被降解的可能性比其他细胞室要大得多。因为在细胞质中含有大量的蛋白酶。另外,从细胞质蛋白混合物中纯化目的蛋白相对比较困难,因为在此细胞室包含总细胞蛋白的绝大部分蛋白[144]。2.细胞外周质表达 在细胞
蛋白系统,该系统由硫氧还蛋白还原酶和硫氧还蛋白组成;谷氧还蛋白系统,该系统由谷胱甘肽还原酶、谷胱甘肽和三种谷氧还蛋白组成[143]。制造次还原细胞质环境,以利于二硫键形成的策略包括选用硫氧还蛋白还原酶(trxB)缺陷的E.coli菌株,这有助于巯基还原势能。 蛋白质在细胞质中被降解的可能性比其他细胞室要大得多。因为在细胞质中含有大量的蛋白酶。另外,从细胞质蛋白混合物中纯化目的蛋白相对比较困难,因为在此细胞室包含总细胞蛋白的绝大部分蛋白[144]。 2.细胞
技术资料暂无技术资料 索取技术资料
谷氧还蛋白3(GLRX3)重组蛋白
询价





