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- DH203-1.1
- Sigma分装
- 2025年07月15日
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文献和实验Culture Systems: Mineral Oil Overlay
Mineral oil overlay microdrop is commonly used during in vitro fertilization (IVF) procedures. Though mineral oil appears homogeneous, it is an product that can vary in quality. Here, we describe the history, chemistry
, its strong adjuvant effect, and the considerable literature describing its activity. The combination of mineral oil (Bayol 55) and dead mycobacterial organisms produces a specific cellular reaction in experimental animals. The mycobacteria in FCA stimulate
-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
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