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鼎国昌盛
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大量
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文献和实验of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2. Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M
SDS Gel Electrophoresis of Tubulin\MAPs
% TCA Procedure Prepare 15 cm glass tubes with 6 mm internal diameter. Polymerize a 10 cm separation gel and a 1 cm stacking gel within the tubes by the addition of TEMED and ammonium persulfate as directed in Chapter Four.
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Acrylamide Gels for DNA): 10.7 ml of 30% Acrylamide 1 ml of 20X TBE 29 ml of ddH2 O 0.2 ml of 10% Ammonium Persulfate (see Hint #7) 60 μl of TEMED 12.After the gel has polymerized, set
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