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文献和实验Purification of a VesicleVacuole (V) Fraction from Aspergillus
and stress response, it is important to understand the composition and function of the membrane-bound organelles that house this biosynthetic machinery. Isolation of vesicles, endosomes, and vacuoles (V fraction) is, therefore, an essential method to study
Isolation and Application of Bioactive Wnt Proteins Methods in Molecular Biology v468. chapter 2 Abstract Wnt proteins and their signaling cascades are involved in a wide variety of developmental processes, andderegulation
). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
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