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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考产品说明书
- 亚型:
IgG
- 形态:
液体
- 保存条件:
2-8°C
- 标记物:
Conjugate / Format
- 适应物种:
Mouse
- 保质期:
请参考产品说明书
- 抗原来源:
请参考产品说明书
- 库存:
大量
- 供应商:
欣博盛
- 宿主:
Donkey
- 应用范围:
ELISA
- 浓度:
请参考产品说明书
- 靶点:
IgG(H+L)
- 抗体英文名:
Donkey F(ab')2 Anti-Mouse IgG(H+L)-HRP
- 抗体名:
Donkey F(ab')2 Anti-Mouse IgG(H+L)-HRP
- 规格:
1.0 mL
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
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![Mouse IgG2a isotype control [MOPC-173] (APC)](https://img1.dxycdn.com/2023/0711/100/6547477506993858761-14.jpg!wh200)

