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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Liquid
- 保存条件:
Store at room temperature.
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX03608
- 级别:
Reagents
- 库存:
Available
- 供应商:
GeneTex
- 应用范围:
IHC-P
- 抗体英文名:
Quatro Sol I 10X Citrate Buffer pH6.0 Kit
- 抗体名:
Quatro Sol I 10X Citrate Buffer pH6.0 Kit
- 规格:
500 ml
IHC-P analysis of human colon adenocarcinoma tissue using GTX20911 p53 antibody [DO-7] (ready-to-use) with GTX03608 Quatro Sol I 10X Citrate Buffer pH6.0 Kit.
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文献和实验Whole mount in situ hybridization with digoxygenin probes
: 10 X Hexanucleotide buffer (or Vial 5 of Genius kit) 2 µl dig-dNTP mix ( Vial 6 of Genius kit ) 2 µl Klenow 1 µl Incubate at 37 C o/n Add 1 µl Klenow and incubate for an additional 4 hr. Stop reaction with : 0.5 M EDTA 2 µl
Southern Blotting: DNA Transfer
NaOH, 1.5 M NaCl (for uncharged membrane). Transfer >3 hours. 2. Crosslink if using an uncharged membrane. Solutions for Southern Hybridization and Development Hybridization 20x SSC: 3M NaCl 0.3M Na-citrate; pH7.0 Hybridization Sol'n: 5x SSC
Southern Blotting(Fred Hutchinson Cancer Research Cente
;3 hours. 2. Crosslink if using an uncharged membrane. Solutions for Southern Hybridization and Development Hybridization 20x SSC: 3M NaCl 0.3M Na-citrate; pH7.0 Hybridization Sol'n: 5x SSC 0.5% (w/v) Blocking Reagent 0.1% (w/v) N
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