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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
干燥、避光、密闭、低温(0-10℃
- 英文名:
EUPALITIN-3-O-GALACTOSIDE
- 库存:
10
- 供应商:
上海乔羽生物
- 规格:
mg/g
货号:QY-YW2706通用名:98604-37-6,3-(beta-D-吡喃半乳糖氧基)-5-羟基-2-(4-羟基苯基)-6,7--4H-1-苯并呋喃-4-酮,EUPALITIN-3-O-GALACTOSIDE英文名称:EUPALITIN-3-O-GALACTOSIDE中文名称:3-(beta-D-吡喃半乳糖氧基)-5-羟基-2-(4-羟基苯基)-6,7--4H-1-苯并呋喃-4-酮CAS:98604-37-6
存储条件:密封避光. 有效期:24个月.(具体来电咨询!) 包装:可根据客户需求提供相应批量包装。 用途:用于含量测定、鉴别、药理实验、活性筛选等。分子结构式:贮藏:干燥、避光、密闭、低温(0-10℃)上海乔羽生物科技有限公司专业供应3-(beta-D-吡喃半乳糖氧基)-5-羟基-2-(4-羟基苯基)-6,7--4H-1-苯并呋喃-4-酮是国内的98604-37-6权威供应商.欢迎致电!
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文献和实验Assay for ß-galactosidase in E. Coli
, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU). We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted
mice and the screen was used to isolate a panel of protease-inhibiting scFvs. Candidate scFvs were validated for inhibitory activity using an o -nitrophenyl-β-galactoside (ONPG) hydrolysis assay. The methods can be used more generally
b-Galactosidase Activity Assay
). Chloroform is easier to pippete if the air in the pippete tip is saturated by drawing up and releasing chloroform several times.Vortex; equilibrate the tubes 5' in a 28 C water bath.AssayStart reaction by adding 0.2 mL substrate, o -nitrophenyl-b -D
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