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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
干燥、避光
- 英文名：
EUPALITIN-3-O-GALACTOSIDE
- 库存：
10
- 供应商：
上海远慕生物
- 规格：
mg/g
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文献和实验Assay for ß-galactosidase in E. Coli
, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU). We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted
mice and the screen was used to isolate a panel of protease-inhibiting scFvs. Candidate scFvs were validated for inhibitory activity using an o -nitrophenyl-β-galactoside (ONPG) hydrolysis assay. The methods can be used more generally
b-Galactosidase Activity Assay
). Chloroform is easier to pippete if the air in the pippete tip is saturated by drawing up and releasing chloroform several times.Vortex; equilibrate the tubes 5' in a 28 C water bath.AssayStart reaction by adding 0.2 mL substrate, o -nitrophenyl-b -D
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