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文献和实验a wedge-shaped piece of teflon held in artery forceps, transfer to Eppendorf tubes and spin them down into a pellet. The presence of the protein in the buffer will stop the cells from sticking to the sides of the centrifuge tube. Cells may be scraped
Coupling Peptides To Resin For Affinity Purification
. Wash the resin twice in buffer by centrifugation. The original resin, and the resin after step 8 should be only lightly labeled, wherease the resin after step five should be heavily labeled. 上一篇:ANTIBODY BINDING TO PROTEIN A AND PROTEIN
The Application of LR Gold Resin for Immunogold Labeling
) or inaccessibility (because of high-density crosslinkage of proteins with the fixatives and resin). Protein integrity may be maintained to a degree by adopting a more minimal fixation regime (e.g., omission of OsO4 [see Note 1 ], decreased concentration of aldehyde
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