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文献和实验Mammalian Brain Acetylcholinesterase
Acetylcholinesterase (acetylcholine acetylhydrolase, AChE: EC 3.1.1.7) has been the focus of much attention since it was first suggested that it plays an important role in the rapid destruction of acetylcholine (ACh) in a living organ (Dale, 1914
Expressing Intrabodies in Mammalian Cells
, they can act as therapeutic molecules for the treatment of viral pathologies, cancer, and misfolding diseases. This chapter provides protocols for application of intrabodies in mammalian cells. We describe all the steps to express intrabodies in different
Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors
In this chapter, the focus is on expression of recombinant mammalian membrane proteins in mammalian host cells, particularly applying Semliki Forest virus (SFV) vectors. Replication-deficient SFV vectors are rapidly generated at high titers in BHK
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