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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
FastDNA® Spin Kit For Feces
- 库存:
大量
- 供应商:
北京智杰方远
- 规格:
50次
北京智杰方远科技有限公司现货供应,欢迎来电详谈!TEL:010-82897201 , 010-62894435;
QQ:523539965 , 2276480926; 手机微信同号:15810200961 , 13521728523; EMail:15810200961@163.com 网址:www.biozj.com
从人类和动物的粪便样品中有效分离基因组DNA,直接用于PCR
1.多达500mg的粪便样品
2.内含配合FastPrep仪 器使用的理解介质E
3.通常可以从500mg的粪便样品中分离得到10-20μg总DNA
4.去除对下游实验有影响的
快速高效提取粪便DNA,可裂解任何粪便样品。可直接用于PCR等其下游实验,配合FastPrep仪器使用无需研磨样品。
The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.
Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
产品应用,如下:
The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
产品属性,如下:
| Product Type |
Kits & Assays |
| Components |
Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each |
| Instrument Compatibility |
FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1 |
| Sample Type |
Soil; Feces; Environmental Water; Wastewater; Sludge |
| Sample Volume |
up to 500 mg per prep |
| Tube Compatibility |
2 mL |
| Matrix Type |
E |
| Molecule Enriched |
DNA |
北京智杰方远科技有限公司成立于2011年是一家细胞培养产品专业供应商,专注细胞生物学试剂开发、销售、服务的高新技术企业。主要代理产品如下: 1.进口胎牛血清:美国GIBCO、德国PAN-biotech、以色列BI、澳大利亚Ausgene-X、美国GEMINI、美国Hyclone等。 2.TAKARA、Biolog微生物鉴定板、Borer清洗产品、台湾旭基。 3.MP Bio、日本同仁、Thermo、SIGMA、QIAGEN、MERCK、LEICA、Millipore、Whatman等。好产品+好价格+好服务为经营理念,希望不断发展的智杰方远成为您最佳的选择!
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文献和实验详情产品名基因组裂解方法纯化得率实验设备 BioTeke Soil Genomic DNA Rapid Extraction Kit 酶法破碎基因组完整特制纯化柱得率纯度高时间1小时内不需要特殊设备FastDNA® SPIN Kit钢珠破碎基因组断裂玻璃奶离心吸附柱纯化得率低时间2小时需要专门破碎设备UltraClean™ Soil DNA Isolation Kit玻璃珠破碎基因组断裂离心吸附柱纯化得率低时间2小时需要专门震荡器玻璃奶纯化得率60-75% ,离心吸附
,每一次循环所产生的DNA均能成为下一次循环的模板,每一次循环都使两条人工合成的引物间的DNA特异区拷贝数扩增一倍,PCR产物得以2n 的批数形式迅速扩增,经过25~30个循环后,理论上可使基因扩增109 倍以上,实际上一般可达106 ~107 倍。 假设扩增效率为“X”,循环数为“n”,则二者与扩增倍数“y”的关系式可表示为:y=(1+X)n 。扩增30个循环即n=30时,若X=100%,则y=230 =1073741824(>109 );而若X=80%时,则y=1.830
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