Recombinant Human EphA4 / HEK8 Protein (aa 570-986, His & GST Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
50 µg
重组人 EphA4 / HEK8 蛋白 (aa 570-986, His & GST标签)(产品说明)
蛋白名称:Human EphA4 / HEK8 Protein (aa 570-986, His & GST Tag)
蛋白构建:A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.2. Measured by its binding ability in a functional ELISA. Immobilized human EPHA4 (aa 570-986) (Cat: 11314-H20B1) at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human EPHA4 /GST chimera consists of 654 amino acids and has a calculated molecular mass of 75 kDa. The recombinant protein migrates as an approximately 67 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P54764
蛋白氨基酸序列:Ser570-Val986
蛋白标签:N-GST & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Wolff, DW; et al. Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4. Cell chemical biology, Pubmed: 35148834