文献支持
AMPK (G1/B2/A1) Heterotrimer重组
蛋白|Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein- ￥4650 - 5820
- Sino Biological
- 北京
- CT005-H0907B
- 2025年08月12日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥4650.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥5820.0 |
重组人 AMPK (G1/B2/A1) 异源三聚体 蛋白（产品说明）
蛋白名称：Human AMPK (G1/B2/A1) Heterotrimer Protein
蛋白构建：A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 94 % as determined by SDS-PAGE
蛋白活性：The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met & Met & His
蛋白分子量：The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号：P54619 & O43741 & Q13131-1
蛋白氨基酸序列：Met1-Pro331 & Met1-Ile272 & Met1-Gln559
蛋白标签：N-GST & N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human AMPK (G1/B2/A1) Heterotrimer Protein
蛋白构建：A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 94 % as determined by SDS-PAGE
蛋白活性：The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met & Met & His
蛋白分子量：The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号：P54619 & O43741 & Q13131-1
蛋白氨基酸序列：Met1-Pro331 & Met1-Ile272 & Met1-Gln559
蛋白标签：N-GST & N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
1, Wang T, et al. The AMPK-HOXB9-KRAS axis regulates lung adenocarcinoma growth in response to cellular energy alterations.Cell reports, PubMed ID: 36001969
技术资料暂无技术资料 索取技术资料
文献支持
AMPK (G1/B2/A1) Heterotrimer重组蛋白|Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein
￥4650 - 5820
