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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human HER3 / ErbB3 Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥3870.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥25190.0 |
蛋白名称:Human HER3 / ErbB3 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the human ErbB3 (NP_001973.2) (Met1-Thr643)was fused with Fc region of mouse IgG at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized human ErbB3-mFc at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (Cat:11609-H01H2), The EC50 of biotinylated human NRG1 (Cat:11609-H01H2) is 0.58-1.25 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 20
蛋白分子量:The recombinant human ErbB3/mFc is a disulfide-linked homodimer. The reduced monomer comprises 858 amino acids and has a predicted molecular mass of 95 kDa. The apparent molecular mass of the protein is approximately 119-129 in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001973.2
蛋白氨基酸序列:Met1-Thr643
蛋白标签:C-mouse IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验photoprotein aequorin by immobilized metal-ion affinity chromatography, Protein Express. Purif. 27 (2003) 384-390. 33. Y. Shi, C. Jiang, Q. Chen, H. Tang, One-step on-column affinity refolding purification and functional analysis of recombinant human VDAC
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
serumfreeconditioned medium (11) ). 3. Methods When expressing recombinant proteins, the choice of vector hasa major impact on purification strategy, yield, and utility of theproduct. Vectors that link a poly-histidine tag to the recombinant protein typically
,EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体,而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体:hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本:蜡包埋人类肺部肿瘤抗原修复缓冲液:柠檬酸盐缓冲液(左)或者 EDTA 缓冲液(右)煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后,才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然,一些研究人员也使用水浴锅,但是,因为该步骤中所选择的装置对染色存在积极或消极的影响
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