重组狨猴 IL-23 (IL23A & IL12B Heterodimer)蛋白

重组狨猴 IL-23 (IL23A & IL12B 异源二聚体) 蛋白（产品说明）
蛋白名称：Marmoset IL-23 (IL23A & IL12B Heterodimer) Protein
蛋白构建：A DNA sequence encoding the marmoset IL23A (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the marmoset IL12B (Met 1-Asn328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A & the marmoset IL12B heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized marmoset IL23A-His+marmoset IL12B-His (Cat:CT037-C08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (Cat:11674-H08H). The EC50 of biotinylated human IL12RB1-His (Cat:11674-H08H) is 55-129 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Arg 20 & Ile 23
蛋白分子量：The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE.
蛋白NP号：0
蛋白氨基酸序列：Met 1-Pro 189 & Met 1-Asn 328
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.