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100 µL
抗体种属:Human
抗体靶点:CD86
抗体应用:FCM
抗原货号:10699-H08H
抗原描述:Recombinant Human CD86 / B7-2 protein (Catalog#10699-H08H)
抗体宿主:Mouse
抗体Ig类型:Mouse IgG1
抗体纯化方法:Protein A
抗体制备:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD86 / B7-2 (rh CD86 / B7-2; Catalog#10699-H08H; NP_008820.2; Met1-His239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human CD86 / B7-2
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验, while the second is provided by the B-cell activation antigens B7-1, or CD80 and B7-2, or CD86 by interaction with the T-cell surface molecules CD28 and CTLA4 Positive staining (normal): interdigitating dendritic cells in T zones of secondary lymphoid organs
B7 家族 白细胞分化抗原及其功能 B7―1和B7-2 B7―1和B7―2分别称CD80和CD86,属B7家族成员。胞膜外区结构属IgSF成员,各有1个V样区和C2区,两者在氨基酸水平上有25%同源性。CD80胞质区较短,富含精氨酸,有1个RRES序列,为钙调蛋白依赖的磷酸化位点;CD86胞质区含有3个PKC磷酸化位点。 在活化的T细胞、B细胞和单核细胞表达水平明显增加。IL-4和TNF-7分别上调B细胞和单核细胞CD86表达
瘤上清(ATCC号TIB120)、B7-2/CD86的杂交瘤上清(Pharmingen)、DEC-205的抗体(ATCC号HB290) 10. 解剖用品 11. 无菌纱布垫 12. 100mm培养皿(如Falcon) 13. 3ml注射器25G,5/8in(1.58cm)针头 14. 9in(23cm)巴氏吸管(填充棉花并高压灭菌) 15. Nytex滤器(3-40/26;Tetko)
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