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- 详细信息
- 文献和实验
- 技术资料
- 库存：
5663
- 供应商：
齐一生物科技（上海）有限公司
- 检测范围：
详细请见说明书
- 检测方法：
ELISA酶联免疫法
- 应用：
科研检测
- 标记物：
见说明
- 样本：
血清，血浆，尿液，胸腹水，脑脊液，细胞培养上清，组织匀浆等
- 规格：
48T/96T
人凝血因子X（FX）ELISA检测试剂盒齐一生物科技（上海）有限公司新品上市促销欢迎您访问我们的网站如有什么问题请给我们电话021-60348496或者留言741653262@qq.com,Web:www.qiyibio.com告诉我您的需要,并留下您宝贵的意见和建议
FOR RESEARCH USE ONLY
Drug Names
Generic Name：人凝血因子X（FX）ELISA检测试剂盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit 48determinations 96 determinations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard：360ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution （20ml×20 fold）
×1bottle （20ml×30 fold）
×1bottle 2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separately (blank comparison wells .don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage： 2-8℃.
2．validity： six months.
人凝血因子X（FX）ELISA检测试剂盒其它产品信息
BRDU IN-SITU DTEC KIT 50SLIDES 550803 美国BD
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HU CD138 PE MAB 100TST DL-101 550805 美国BD
HU BLD GROUP A PURE MAB 0.1MG NAM87-1F6 550806 美国BD
HU BLD GROUP A FITC MAB 0.1MG NAM87-1F6 550807 美国BD
HU LAIR-1 PURE MAB 0.1MG DX26 550810 美国BD
HU LAIR-1 PE MAB 100TST DX26 550811 美国BD
HU CD244 PURE MAB 0.1MG 2-69 550814 美国BD
HU CD244 FITC MAB 100TST 2-69 550815 美国BD
HU CD244 PE MAB 100TST 2-69 550816 美国BD
ACT CASPASE-3 PE MAB 100TST C92-605 550821 美国BD
PI/RNASE STAINING BUF 100ML 550825 美国BD
MS IG APC PAB 0.1MG POLY1270 550826 美国BD
HU P16 IHC MAB 1.0ML G175-405 550834 美国BD
MLH-1 IHC PURE MAB 1.0ML G168-15 550838 美国BD
HPV-16 L1 IHC PURE MAB 1.0ML CAMVIR-1 550840 美国BD
HU ESPNL MBP IHC PURE MAB 1.0ML AHE-2 550843 美国BD
HU CD11A NHP PE MAB 50TST HI111 550851 美国BD
HU CD11A NHP APC MAB 50TST HI111 550852 美国BD
HU HLA-DR DP DQ NHP FITC MAB 50TST TU39 550853 美国BD
MS IGG1 KPA ITCL NHP APC 50TST MOPC-21 550854 美国BD
HU CD45RA APC MAB 100TST HI100 550855 美国BD
HU CD195 APC MAB 100TST 3A9 550856 美国BD
HU A20 PURE MAB 50UG E5-1619 550859 美国BD
MS KLRG1 BIOTIN MAB 0.1MG 2F1 550863 美国BD
HU CD62P NHP FITC MAB 50TST AC1.2 550866 美国BD
HU CD16B PE MAB 100TST CLB-GRAN11.5 550868 美国BD
MS IGG1 APC MAB 0.1MG X56 550874 美国BD
RBT IGG STD PURE PAB 0.1MG POLY1281 550875 美国BD
MS IGG1 KPA ITCL IHC PURE 1.0ML MOPC-31C 550878 美国BD
DAB SBSTR KIT 550880 美国BD
MS IGM PERCP-CY5.5 MAB 0.1MG R6-60.2 550881 美国BD
MS IGG2A KPA ITCL APC .1MG G155-178 550882 美国BD
MS IGM KPA ITCL APC 0.1MG G155-228 550883 美国BD
RAT IGG1 LAM ITCL APC MAB 0.1MG A110-1 550884 美国BD
HU CD62P APC MAB 100TST AK-4 550888 美国BD
HU CD206 APC MAB 100TST 19.2 550889 美国BD
HU CD137 APC MAB 100TST 4B4-1 550890 美国BD
BRDU BUF 25MG 550891 美国BD
HU CD120A BIOTIN MAB 0.5MG MABTNFR1-B1 550900 美国BD
HU CDW329 FITC MAB 100TST E10-286 550906 美国BD
ANXN V-FITC FLUORESCENCE MICROSCOPY KIT 550911 美国BD
HU CD253 NALE MAB 0.25MG RIK-2 550912 美国BD
HU CYCLIN A PE SET 100TST 550913 美国BD
ACT CASPASE-3 APOP PE KIT 100TST 550914 美国BD
HU CDC25C PURE MAB 50UG TC113 550920 美国BD
MS IGA KPA ITCL PURE MAB 0.5MG MOPC-320 550924 美国BD
HU IP-10 OPTEIA SET 20TST 550926 美国BD
MS IGG2A ITCL PERCP-CY5.5 0.1MG G155-178 550927 美国BD
HU ACT CASP-3 OPTEIA SET 5TST 550930 美国BD
HU IGG APC MAB 100TST G18-145 550931 美国BD
HU (CD197) CCR7 PURE MAB 0.25MG 2H4 550937 美国BD
RBT IGM BIOTIN MAB 0.2MG 367.2 550938 美国BD
HU UBIQUITIN ASCI 100UL 6C1.17 550944 美国BD
SAV-HRP PRE-DILUTED 50ML 550946 美国BD
HU C4A OPTEIA KIT 1PLT 550947 美国BD
HU CD253 OPTEIA SET 20TST 550948 美国BD
MS IL-6 OPTEIA KIT 2PLT 550950 美国BD
HU CTKN 14-15-16-19 PURE MAB 0.1MG KA4 550951 美国BD
CYTOKERATIN 14,15,16,19 PE SET 100TST 550953 美国BD
MS CD4 PERCP-CY5.5 MAB 0.1MG RM4-5 550954 美国BD
HU CD79B APC MAB 100TST CB3-1 550955 美国BD
HU CD36 APC MAB 100TST CB38 550956 美国BD
JURKAT APOPTOTIC LYSATE SET I 1.0MG 550959 美国BD
RAT ERYTRD CELLS PURE MAB 0.1MG HIS49 550961 美国BD
RAT ERYTRD CELLS BIOTIN MAB 0.1MG HIS49 550962 美国BD
MS IGM ITCL PURE MAB 0.5MG PMG1-146.1 550963 美国BD
MS CD72ABD ALOATG FITC MAB 0.1MG K10.6 550966 美国BD
HU CD183 APC MAB 100TST 1C6/CXCR3 550967 美国BD
HU CD161 APC MAB 100TST DX12 550968 美国BD
RAT CD8B BIOTIN MAB 0.1MG 341 550970 美国BD
RAT CD11A PE MAB 0.1MG WT.1 550972 美国BD
RAT CD28 FITC MAB 0.1MG JJ319 550973 美国BD
RAT CD44H FITC MAB 0.1MG OX-49 550974 美国BD
RAT CD59 FITC MAB 0.1MG TH9 550976 美国BD
RAT CD134 BIOTIN MAB 0.1MG OX-40 550977 美国BD
RAT CD161A BIOTIN MAB 0.1MG 10/78 550978 美国BD
RAT CD6 PAN-T CELLS PURE MAB 0.1MG OX-52 550979 美国BD
RAT RT1 AAB FITC MAB 0.1MG C3 550981 美国BD
RAT RT1D FITC MAB 0.1MG OX-17 550982 美国BD
MS LY-49F BIOTIN MAB 0.1MG HBF-719 550986 美国BD
MS LY-49F PE MAB 0.1MG HBF-719 550987 美国BD
HU CD44 PURE MAB 0.1MG 515 550988 美国BD
HU CD44 PE MAB 100TST 515 550989 美国BD
HU CD44 NALE MAB 0.5MG 515 550990 美国BD
MS CD19 APC MAB 0.1MG 1D3 550992 美国BD
MS CD11B PERCP-CY5.5 MAB .1MG M1/70 550993 美国BD
MS CD45 PERCP-CY5.5 MAB 0.1MG 30-F11 550994 美国BD
HU LT-ALP OPTEIA SET 20TST 550995 美国BD
HU MIG OPTEIA SET 20TST 550998 美国BD
HU IL-8 OPTEIA KIT II 2PLT 550999 美国BD
MS CD19 PERCP-CY5.5 MAB 0.1MG 1D3 551001 美国BD
SAV-AKP PRE-DILUTED 50ML 551008 美国BD
MS IG HRP DTEC KIT 551011 美国BD
HAMSTER IG HRP DTEC KIT 551012 美国BD
ANTI-RAT IG HRP DTEC KIT 551013 美国BD
AEC SBSTR KIT 500TST 551015 美国BD
MS IL-4 BD ELISPOT SET 10PLT 551017 美国BD
HU IL-10 BD ELISPOT SET 10PLT 551018 美国BD
RAT IG APC PAB 0.1MG POLY1271 551019 美国BD
BCL-X PURE MAB W/CONT 50UG 2H12 551020 美国BD
BCL-X PURE MAB W/CONT 150UG 2H12 551022 美国BD
HU PARP PURE MAB W/CONT 50UG 7D3-6 551024 美国BD
HU PARP PURE MAB W/CONT 150UG 7D3-6 551025 美国BD
FOR RESEARCH USE ONLY
Drug Names
Generic Name：人凝血因子X（FX）ELISA检测试剂盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit 48determinations 96 determinations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard：360ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution （20ml×20 fold）
×1bottle （20ml×30 fold）
×1bottle 2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separately (blank comparison wells .don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage： 2-8℃.
2．validity： six months.
人凝血因子X（FX）ELISA检测试剂盒其它产品信息
BRDU IN-SITU DTEC KIT 50SLIDES 550803 美国BD
HU CD138 PURE MAB 0.1MG DL-101 550804 美国BD
HU CD138 PE MAB 100TST DL-101 550805 美国BD
HU BLD GROUP A PURE MAB 0.1MG NAM87-1F6 550806 美国BD
HU BLD GROUP A FITC MAB 0.1MG NAM87-1F6 550807 美国BD
HU LAIR-1 PURE MAB 0.1MG DX26 550810 美国BD
HU LAIR-1 PE MAB 100TST DX26 550811 美国BD
HU CD244 PURE MAB 0.1MG 2-69 550814 美国BD
HU CD244 FITC MAB 100TST 2-69 550815 美国BD
HU CD244 PE MAB 100TST 2-69 550816 美国BD
ACT CASPASE-3 PE MAB 100TST C92-605 550821 美国BD
PI/RNASE STAINING BUF 100ML 550825 美国BD
MS IG APC PAB 0.1MG POLY1270 550826 美国BD
HU P16 IHC MAB 1.0ML G175-405 550834 美国BD
MLH-1 IHC PURE MAB 1.0ML G168-15 550838 美国BD
HPV-16 L1 IHC PURE MAB 1.0ML CAMVIR-1 550840 美国BD
HU ESPNL MBP IHC PURE MAB 1.0ML AHE-2 550843 美国BD
HU CD11A NHP PE MAB 50TST HI111 550851 美国BD
HU CD11A NHP APC MAB 50TST HI111 550852 美国BD
HU HLA-DR DP DQ NHP FITC MAB 50TST TU39 550853 美国BD
MS IGG1 KPA ITCL NHP APC 50TST MOPC-21 550854 美国BD
HU CD45RA APC MAB 100TST HI100 550855 美国BD
HU CD195 APC MAB 100TST 3A9 550856 美国BD
HU A20 PURE MAB 50UG E5-1619 550859 美国BD
MS KLRG1 BIOTIN MAB 0.1MG 2F1 550863 美国BD
HU CD62P NHP FITC MAB 50TST AC1.2 550866 美国BD
HU CD16B PE MAB 100TST CLB-GRAN11.5 550868 美国BD
MS IGG1 APC MAB 0.1MG X56 550874 美国BD
RBT IGG STD PURE PAB 0.1MG POLY1281 550875 美国BD
MS IGG1 KPA ITCL IHC PURE 1.0ML MOPC-31C 550878 美国BD
DAB SBSTR KIT 550880 美国BD
MS IGM PERCP-CY5.5 MAB 0.1MG R6-60.2 550881 美国BD
MS IGG2A KPA ITCL APC .1MG G155-178 550882 美国BD
MS IGM KPA ITCL APC 0.1MG G155-228 550883 美国BD
RAT IGG1 LAM ITCL APC MAB 0.1MG A110-1 550884 美国BD
HU CD62P APC MAB 100TST AK-4 550888 美国BD
HU CD206 APC MAB 100TST 19.2 550889 美国BD
HU CD137 APC MAB 100TST 4B4-1 550890 美国BD
BRDU BUF 25MG 550891 美国BD
HU CD120A BIOTIN MAB 0.5MG MABTNFR1-B1 550900 美国BD
HU CDW329 FITC MAB 100TST E10-286 550906 美国BD
ANXN V-FITC FLUORESCENCE MICROSCOPY KIT 550911 美国BD
HU CD253 NALE MAB 0.25MG RIK-2 550912 美国BD
HU CYCLIN A PE SET 100TST 550913 美国BD
ACT CASPASE-3 APOP PE KIT 100TST 550914 美国BD
HU CDC25C PURE MAB 50UG TC113 550920 美国BD
MS IGA KPA ITCL PURE MAB 0.5MG MOPC-320 550924 美国BD
HU IP-10 OPTEIA SET 20TST 550926 美国BD
MS IGG2A ITCL PERCP-CY5.5 0.1MG G155-178 550927 美国BD
HU ACT CASP-3 OPTEIA SET 5TST 550930 美国BD
HU IGG APC MAB 100TST G18-145 550931 美国BD
HU (CD197) CCR7 PURE MAB 0.25MG 2H4 550937 美国BD
RBT IGM BIOTIN MAB 0.2MG 367.2 550938 美国BD
HU UBIQUITIN ASCI 100UL 6C1.17 550944 美国BD
SAV-HRP PRE-DILUTED 50ML 550946 美国BD
HU C4A OPTEIA KIT 1PLT 550947 美国BD
HU CD253 OPTEIA SET 20TST 550948 美国BD
MS IL-6 OPTEIA KIT 2PLT 550950 美国BD
HU CTKN 14-15-16-19 PURE MAB 0.1MG KA4 550951 美国BD
CYTOKERATIN 14,15,16,19 PE SET 100TST 550953 美国BD
MS CD4 PERCP-CY5.5 MAB 0.1MG RM4-5 550954 美国BD
HU CD79B APC MAB 100TST CB3-1 550955 美国BD
HU CD36 APC MAB 100TST CB38 550956 美国BD
JURKAT APOPTOTIC LYSATE SET I 1.0MG 550959 美国BD
RAT ERYTRD CELLS PURE MAB 0.1MG HIS49 550961 美国BD
RAT ERYTRD CELLS BIOTIN MAB 0.1MG HIS49 550962 美国BD
MS IGM ITCL PURE MAB 0.5MG PMG1-146.1 550963 美国BD
MS CD72ABD ALOATG FITC MAB 0.1MG K10.6 550966 美国BD
HU CD183 APC MAB 100TST 1C6/CXCR3 550967 美国BD
HU CD161 APC MAB 100TST DX12 550968 美国BD
RAT CD8B BIOTIN MAB 0.1MG 341 550970 美国BD
RAT CD11A PE MAB 0.1MG WT.1 550972 美国BD
RAT CD28 FITC MAB 0.1MG JJ319 550973 美国BD
RAT CD44H FITC MAB 0.1MG OX-49 550974 美国BD
RAT CD59 FITC MAB 0.1MG TH9 550976 美国BD
RAT CD134 BIOTIN MAB 0.1MG OX-40 550977 美国BD
RAT CD161A BIOTIN MAB 0.1MG 10/78 550978 美国BD
RAT CD6 PAN-T CELLS PURE MAB 0.1MG OX-52 550979 美国BD
RAT RT1 AAB FITC MAB 0.1MG C3 550981 美国BD
RAT RT1D FITC MAB 0.1MG OX-17 550982 美国BD
MS LY-49F BIOTIN MAB 0.1MG HBF-719 550986 美国BD
MS LY-49F PE MAB 0.1MG HBF-719 550987 美国BD
HU CD44 PURE MAB 0.1MG 515 550988 美国BD
HU CD44 PE MAB 100TST 515 550989 美国BD
HU CD44 NALE MAB 0.5MG 515 550990 美国BD
MS CD19 APC MAB 0.1MG 1D3 550992 美国BD
MS CD11B PERCP-CY5.5 MAB .1MG M1/70 550993 美国BD
MS CD45 PERCP-CY5.5 MAB 0.1MG 30-F11 550994 美国BD
HU LT-ALP OPTEIA SET 20TST 550995 美国BD
HU MIG OPTEIA SET 20TST 550998 美国BD
HU IL-8 OPTEIA KIT II 2PLT 550999 美国BD
MS CD19 PERCP-CY5.5 MAB 0.1MG 1D3 551001 美国BD
SAV-AKP PRE-DILUTED 50ML 551008 美国BD
MS IG HRP DTEC KIT 551011 美国BD
HAMSTER IG HRP DTEC KIT 551012 美国BD
ANTI-RAT IG HRP DTEC KIT 551013 美国BD
AEC SBSTR KIT 500TST 551015 美国BD
MS IL-4 BD ELISPOT SET 10PLT 551017 美国BD
HU IL-10 BD ELISPOT SET 10PLT 551018 美国BD
RAT IG APC PAB 0.1MG POLY1271 551019 美国BD
BCL-X PURE MAB W/CONT 50UG 2H12 551020 美国BD
BCL-X PURE MAB W/CONT 150UG 2H12 551022 美国BD
HU PARP PURE MAB W/CONT 50UG 7D3-6 551024 美国BD
HU PARP PURE MAB W/CONT 150UG 7D3-6 551025 美国BD
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文献和实验相关实验
葡萄糖来源 AGEs ELISA 检测试剂盒 （ Glucose-derived AGEs ELISA Kit ） 研 究 背 景 越来越多的证据指出晚期糖基化终端产物（ AGEs ）修饰蛋白严重影响到老龄化人群的健康问题。 AGEs 是葡萄糖与自由的蛋白质氨基基团发生非酶促糖基化反应的终端不可逆代谢产物的总称。其在人体内的积累会引发组织或器官一系列的病理性变化，从而导致白内障、阿尔茨海默氏病、骨关节炎、心功
1、实验目的： 采用德国IBL的EB病毒不同抗原的抗体检测试剂盒对不同感染阶段的病人样本进行检测，统计出，不同阶段各种抗体的阳性率。为临床EB病毒的诊断，特别是EB病毒的免疫学诊断提供方法和策略依据。 2、材料与方法 为了确定IBL EBV ELISA试剂盒的临床灵敏度和特异性，我们总共做了242个样品。其中的样品都取自EBV感染的不同阶段。采用德国IBL的EB病毒不同抗原的抗体检测试剂盒的名称和目录号为： （1）BE病毒壳抗原IgM抗体ELISA检测试剂盒（EBV VCA IgM
：分别是竞争性蛋白质结合分析法[3]、放射性免疫分析法[4]和薄层色谱法[5]。在80年代，放射性免疫分析法得到了广泛的应用和改进，由于这种方法采用放射性核素标记，应用范围受到一定限制，为了进一步提高检测的灵敏度和操作的安全性，进入90年代又陆续发明了各种酶标记法和化学发光法的竞争性ELISA检测试剂盒，这一类检测方法的根本原理都是基于抗体抗原的免疫反应，所有这些试剂盒中采用的抗体全部是兔抗血清或者是经过特异亲和提纯之后的抗cAMP或cGMP的兔多抗，毫无疑问，兔多抗在约四十年的cAMP，cGMP定量
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人凝血因子X（FX）ELISA检测试剂盒
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