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20℃
- 保质期:
12个月
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大量
- 供应商:
信裕生物
- 规格:
0.5 ml
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文献和实验Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Conjugation of Antibodies to Horseradish Peroxidase
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups
Peroxidase Conjugation by Periodate Method
Reagents Horseradish peroxidase Sodium periodate, 0.1M, freshly made Ethylene glycol Sephadex G-25 Sodium acetate buffer, 0.001M, pH 4.2 IgG Carbonate:Bicarbonate Buffer, 1.0 M, pH 9.5 Sodium tetraborate (4 mg/ml) freshly
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