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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
信裕生物
- 检测范围:
见说明书
- 检测方法:
Western blot/Elisa/IHC
- 应用:
科学研究
- 适应物种:
Human/Mouse/Rat
- 标记物:
见说明书
- 样本:
血清液/组织/尿
- 规格:
25 ug
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文献和实验the column with 3 l of Buffer D using a fast flow rate. 2. Elute the column with a 4 l gradient from 100 mM KCl to 500 mM KCl in Buffer D. Collect 25 ml fractions (400 drops/fraction). Actin-containing fractions are identified by dot blot using mAb C4
LABELING MUSCLE ACTIN WITH IATR
. Procedure 1.Equilibrate G-25 column with Buffer 1. 2.Clarify dialyzed actin in a 50Ti rotor for 1 hr at 40,000 rpm,4℃. 3.Run supernatant through the G-25 column,collect 10 drop fractions. 4.Collect fluorescent fractions in the void volume,measure
Labeling Muscle Actin with 5-iodoacetamidofluorescein
solution to actin and mix gently with a Pasteur pipte. 8.Let sit on ice for 2 hr. 9.Stop the reaction by adding DTT to 10 mM. 10.Dialyze against 1 liter buffer 3 overnight. Day 2 Materials 1.Buffer 1 as for day 1,4℃,2000 ml. 2.G-25-150 column,~30
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