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- 2025年08月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
远慕生物
- 检测范围：
科研实验
- 检测方法：
双抗体夹心酶联免疫法(ELISA)
- 样本：
血液、尿，粪便，脑脊液，胸腹水，前列腺液，精液，阴道分泌物等
- 规格：
96T/48T
人血红蛋白C(HbC)ELISA试剂盒说明书
上海远慕生物做实验试剂，专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂！欢迎来电咨询订购：Elisa试剂盒现货低价！特价促销！
Human Hemoglobin C,HbC ELISA Kit
产品货号：YM-KJ1103
规格：96T/48T
欢迎来电咨询订购：Elisa试剂盒，人血红蛋白C(HbC)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人血红蛋白C(HbC)ELISA试剂盒 试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围：
0.2IU/L - 6IU/L
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文献和实验书太大了，上传不了，需要的话我可以发到邮箱或者各位在网上载，assaydesigns公司网页就有。 licanming 怎么无人回啊？只好自己顶一下！ zhidanguilai 检测PKC活性可以用加拿大Immunechem公司的非放射性蛋白激酶C活性分析试剂盒，安全、快速、稳定，可用于筛选蛋白激酶C抑制剂或激活剂、定性定量分析纯化或部分纯化的PKC激酶活性。 测试盒原理： 基于ELISA的基本
人 血红蛋白 （ Hb ) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中血红蛋白（Hb) 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人血红蛋白（Hb) 水平。用纯化的人血红蛋白（Hb) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血红蛋白（Hb) ，再与HRP 标记的血红蛋白（Hb) 抗体结合，形成抗体- 抗原- 酶标
Nucleation and Crystal Growth of Hemoglobins: The Case of HbC
Hemoglobin (Hb) crystallization is of significance both in vivo and in vitro. Hb crystals form in red blood cells (RBCs), as occurs in the case of patients expressing βC -globin (β6 Glu→Lys). In vitro, high-resolution structural determination
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