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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人杀伤细胞凝集素样受体(KLR)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human killer cell lectin-like receptor,KLR ELISA Kit
产品货号:YM-KJ1055
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人杀伤细胞凝集素样受体(KLR)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人杀伤细胞凝集素样受体(KLR)ELISA试剂盒 试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验杀伤细胞凝集素样受体( KLR ) 属C型凝集素家族成员,为Ⅱ型膜蛋白,由CD94和C型凝集素家族不同成员组成。CD94/NKG2A和CD94/NKG2B,由CD94和NKG2A或NKG2B以二硫键连接,两条肽链的膜外区各有一个C型凝集素样(C-lectin)结构域。其中NKG2A和NKG2B分子胞质区有2个ITIM;CD94无此结构,但起着分子伴侣的作用,对CD94/NKG2A的表达及功能行使是不可少的。 CD94/NKG
因子 >>> 分装 ELISA 试剂盒 货号 品名 方法 规格 价格 备注 F00010 血管紧张素转化酶 (Human ACE) ELISA 48T/96T 2000/3000 进口分装 F00020 激活素 A(Activin A) ELISA 48T/96T 2000/3000 进口分装 F00021 雄激素结合蛋白 (ABP) ELISA 48T/96T 1800/2800 进口分装 F00022 促肾上腺皮质激素 (ACTH) ELISA 48T/96
自然杀伤细胞抑制性受体和激活性受体 迄今已发现有数十种NK细胞受体,分属抑制性受体和激活性受体两大类,各大类又包括数个家族,体现了NK细胞受体的多样性。 NK细胞受体包括免疫球蛋白超家族和C型凝集素超家族,每一家族又各包含抑制性受体和活化性受体。小鼠中最早发现NK细胞表面有针对MHCI类分子的抑制性受体,之后有关抑制性受体的研究工作很快扩展到人。这类受体的胞内段均有ITIM,可招募蛋白酪氨酸磷酸酶SHP/SHD―2,介导免疫抑制。表达此类受体的基因在染色体上形成一个基因复合
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