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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人NOD样受体(NLR)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human NOD-like receptor9,NLR ELISA Kit
产品货号:YM-KJ1041
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人NOD样受体(NLR)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人NOD样受体(NLR)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验Immunity:厦门大学韩家淮团队揭示 PELO 控制 NOD 样受体家族蛋白寡聚化组装和激活的机制
在免疫反应起始阶段,机体通过模式识别受体感应病原体相关模式分子 (pathogen associated molecular pattern, PAMP) 和损伤相关模式分子 (damage associated molecular pattern, DAMP) 。 NOD 样受体(Nod-like receptor, NLR)家族是机体最大的一类模式识别受体;目前认为 NLR 感知识别相应模式分子后,可通过自身寡聚化组装形成大型信号分子机器, 如 NLRP3,NLRC4 等形成
NLR 的结构与分类 NLR指NOD样受体(NOD-like receptor)或NACHT-亮氨酸重复序列受体(NACHT-LRR receptor)。受体分子类功能不同的结构域组成。 (1)N端为亮氨酸重复序列(LRR),这是一个与Toll样受体胞外段相同的结构域,专司与配体即PAMP中相应成 (2)分子中段为NLR各成员共有的特征性结构域,称为NACHT。NACHT从下列相关分子缩写词前端l、2叶字母拼接而来:NAIP(神经元凋亡抑制蛋白)、CⅡ
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人Toll 样受体 -3 ( TLR-3 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人TLR-3 单抗包被于酶标板上,标准品和样品中的 TLR-3与单抗结合,加入生物
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