Horse Angiotensinogen(AGT) ELI

Horse Angiotensinogen(AGT) ELISA kit

PRINCIPLE OF THE ASSAY
The microtiter plate provided in this kit has been pre-coated with
Angiotensinogen(AGT). Standards or samples are then added to
the appropriate microtiter plate wells with Horseradish
Peroxidase (HRP) -conjugated antibody preparation specific for
Angiotensinogen(AGT)，mix well and incubated. The more the
amount of Angiotensinogen(AGT) in samples, the less HRP-
conjugated antibody preparation specific for Angiotensinogen
(AGT) bound by pre-coated Angiotensinogen(AGT). Then a TMB
(3,3',5,5' tetramethyl-benzidine) substrate solution is added to
each well. And the color develops in opposite to the amount of
Angiotensinogen(AGT) in the sample. The color development is
stopped and the intensity of the color is measured.

Horse Angiotensinogen(AGT) ELISA kit

SPECIFICITY
This assay recognizes horse Angiotensinogen(AGT). No
significant cross-reactivity or interference was observed.

Horse Angiotensinogen(AGT) ELISA kit

3
SENSITIVITY
The minimum detectable dose of horse Angiotensinogen(AGT) is
typically less than 15.6 pg/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD)
was defined as the lowest protein concentration that could be
differentiated from zero.
MATERIALS PROVIDED
Reagent Quantity
Assay plate 1
Standard
1 x 100 μl
(A solution of 20000 pg/ml)
Sample Diluent 2 x 20 ml
HRP- conjugate Diluent 1 x 10 ml
HRP- conjugate 1 x 30 μl
Wash Buffer
1 x 20 ml
(25×concentrate)
TMB Substrate 1 x 10 ml
Stop Solution 1 x 10 ml

Horse Angiotensinogen(AGT) ELISA kit