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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Mouse diamine oxidase,DAO ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
1000 2.879 2.752 2.816 2.607
500 2.301 2.216 2.259 2.050
250 1.584 1.560 1.572 1.363
125 0.945 0.932 0.939 0.730
62.5 0.655 0.672 0.664 0.455
31.2 0.532 0.542 0.537 0.328
15.6 0.412 0.407 0.410 0.201
0 0.211 0.206 0.209
Mouse diamine oxidase,DAO ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Mouse diamine oxidase,DAO ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of mouse DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 84
Range % 81-87
1:2 Average % 95
Range % 91-99
1:4 Average % 91
Range % 88-96
1:8 Average % 96
Range % 93-99
Mouse diamine oxidase,DAO ELISA Kit
回收率:The recovery of mouse DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 109 104-112
EDTA plasma (n=4) 85 81-89
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Methods for the Detection of D-Amino-Acid Oxidase
that the mouse does not have this enzyme in its liver (13 ). The mouse is a very unique animal in this respect. Northern hybridization, RT-PCR, and western blotting gave positive results for the presence of D-amino-acid oxidase in the ddY/DAO- mice (12 , 17
Methods for the Detection of D-Amino-Acid Oxidase
( 2 , 3 ). In previous work, my colleagues and I concluded that D-amino-acid oxidase is not present in the mouse liver ( 13 ). This paper examines in greater detail the methods used in reaching that conclusion. 4 ). Organs, tissues, or tissue culture cells
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