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- xy-291Mu02
- 2025年07月13日
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- 详细信息
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Nei Endonuclease VIII Like Protein 3 (NEIL3)
- 库存：
1000
- 供应商：
上海信裕
Nei内切核酸酶Ⅷ样蛋白3(NEIL3)重组蛋白
Recombinant Nei Endonuclease VIII Like Protein 3 (NEIL3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | xy291Mu02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | >95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
Nei内切核酸酶Ⅷ样蛋白3(NEIL3)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Nei内切核酸酶Ⅷ样蛋白3(NEIL3)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Nei内切核酸酶Ⅷ样蛋白3(NEIL3)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Nei内切核酸酶Ⅷ样蛋白3(NEIL3)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
酶活性 二、机制 目前普遍认为,共抑制、基因压制和RNAi很可能具有相同的分子机制,都是通过dsRNA的介导而特异地降解靶mRNA, 抑制相应基因的表达. 即RNAi、共抑制、quelling均属于PTGS！现已初步阐明dsRNA介导的同源性靶mRNA降解过程主要分为两步. 第一步（起始阶段）是较长ds RNA在ATP参与下被RNaseⅢ样的特异核酸酶切割加工成21~23nt的由正义和反义链组成的小干扰RNA（small interfering RNA,siRNA）. 第二步（效应阶段
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Nei内切核酸酶Ⅷ样蛋白3(NEIL3)重组蛋白
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