Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH‑3T3 mouse embryonic fibroblast cells. rhTenascin-C immobilized at 15 μg/mL, in the presence of 0.1 μg/mL human Fibronectin, will block approximately 70%-90% NIH3/T3 cell adhesion (5 x 104 cells/well, 100 μL/well).
Source
Mouse myeloma cell line, NS0-derived human Tenascin C protein
Gly23-Pro625, with a C-terminal 6-His tag
N-terminal Sequence
Analysis
Gly23
Predicted Molecular Mass
65.3 kDa
SDS-PAGE
97 kDa, reducing conditions