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- xy-205Ra01
- 2025年07月10日
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- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)
- 库存：
1000
- 供应商：
上海信裕
血小板反应蛋白解整合素金属肽酶5(ADAMTS5)重组蛋白
Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy205Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | >95% |
| UOM | 50ug |
| Predicted Molecular Mass | 42.9kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
血小板反应蛋白解整合素金属肽酶5(ADAMTS5)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
RQVE LLLVADSSMA KMYGRGLQHY LLTLASIANR LYSHASIENH IRLAVVKVVV LTDKSLEVSK NAATTLKNFC KWQHQHNQLG DDHEEHYDAA ILFTREDLCG HHSCDTLGMA DVGTICSPER SCAVIEDDGL HAAFTVAHEI GHLLGLSHDD SKFCEENFGS TEDKRLMSSI LTSIDASKPW SKCTSATITE FLDDGHGNCL LDVPRKQILG PEELPGQTYD ATQQCNLTFG PEYSVCPGMD VCARLWCAVV RQGQMVCLTK KLPAVEGTPC GKGRICLQGK CVDKTKKKYY STSSHGNWGS WGPWGQCSRS CGGGVQFAYR HCNNPAPRNS GRYCTGKRAI YRSCSVIPCP PN
RQVE LLLVADSSMA KMYGRGLQHY LLTLASIANR LYSHASIENH IRLAVVKVVV LTDKSLEVSK NAATTLKNFC KWQHQHNQLG DDHEEHYDAA ILFTREDLCG HHSCDTLGMA DVGTICSPER SCAVIEDDGL HAAFTVAHEI GHLLGLSHDD SKFCEENFGS TEDKRLMSSI LTSIDASKPW SKCTSATITE FLDDGHGNCL LDVPRKQILG PEELPGQTYD ATQQCNLTFG PEYSVCPGMD VCARLWCAVV RQGQMVCLTK KLPAVEGTPC GKGRICLQGK CVDKTKKKYY STSSHGNWGS WGPWGQCSRS CGGGVQFAYR HCNNPAPRNS GRYCTGKRAI YRSCSVIPCP PN
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
血小板反应蛋白解整合素金属肽酶5(ADAMTS5)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
血小板反应蛋白解整合素金属肽酶5(ADAMTS5)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
血小板反应蛋白解整合素金属肽酶5(ADAMTS5)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
。胞浆内蛋白在蛋白酶作用下降解成肽段，依次被热休克蛋白HSP70和HSP90传递给膜上的TAP (tansporter associated with antigen processing) ，同时，在蛋白酶的作用下进一步被剪切成小肽。 小肽由TAP经HSP96传递给内质网内新合成的MHC I类分子上，最后由MHC I类分子将结合的抗原小肽托出细胞膜外。细胞外物质经专职APC吞噬（phagocytosis，指颗粒抗原）、胞饮（pinocytosis, 指可溶性抗原）或内吞（endocytosis
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血小板反应蛋白解整合素金属肽酶5(ADAMTS5)重组蛋白
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