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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A)
- 库存:
1000
- 供应商:
上海信裕
Mg2+/Mn2+依赖性蛋白磷酸酶1A(PPM1A)重组蛋白
Recombinant Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy326Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | >95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
Mg2+/Mn2+依赖性蛋白磷酸酶1A(PPM1A)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Mg2+/Mn2+依赖性蛋白磷酸酶1A(PPM1A)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Mg2+/Mn2+依赖性蛋白磷酸酶1A(PPM1A)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Mg2+/Mn2+依赖性蛋白磷酸酶1A(PPM1A)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
培养的各种培养基内都含有必需氨基酸。 2、单糖:培养中的细胞可以进行有氧与无氧酵解,六碳糖是主要能源。此外六碳糖也是合成某些氨基酸、脂肪、核酸的原料。细胞对葡萄糖的吸收能力最高,半乳糖最低。 体外培养动物细胞时,几乎所有的培养基或培养液中都以葡萄糖作为必含的能源物质。 3、维生素:主要扮演辅酶、辅基的角色,必不可少。生物素、叶酸、烟酰胺、泛酸、吡哆醇、核黄素、硫胺素、维生素B12都是培养基常有的成分。 4、无机离子与微量元素:细胞生长除需要钠、钾、钙、镁、氮和磷等基本元素,还需要微量
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Mg2+/Mn2+依赖性蛋白磷酸酶1A(PPM1A)重组蛋白
¥1 - 3980






