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文献和实验Too many bands on a Western blot
(less than 0.2% of total protein), it may be difficult to detect (for instance, synaptobrevin/VAMP comigrates with histones in cell homogenates which interfere with its detection). Signal enhancement may then lead to the appearance of artificial bands. Enrichment
, their active moiety (the light chain) is translocated from the endocytic compartment into the cytosol. Here, it cleaves one among three synaptic proteins (viz. the vesicle-associated membrane protein [VAMP]/synaptobrevin, syntaxin, and synaptosomal-associated
Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin
that is induced by learning, which subsequently alters the processing and response of the nervous system to the sensory information that is learned. Synapto-pHluorin (spH), a protein fusion of a pH-sensitive mutant of green fluorescent protein with synaptobrevin
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