Produced from sera of rabbits immunized with highly pure recombinant Human TFF-2. Anti-Human TFF-2 specific antibody was purified by affinity chromatography and then biotinylated.
Direct To detect Human TFF-2 by direct ELISA (using 100 μl/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TFF-2.
Sandwich To detect Human TFF-2 by sandwich ELISA (using 100 μl/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti- Human TFF-2 (500-P312) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TFF-2.