Zymoclean Large Fragment DNA R

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

), where we have overcome both the size limitations and the restrictions of the cloning/subcloning procedures used in the Red/ET recombineering system. We describe the transfer of a very large DNA fragment (~55 kb) from one BAC into another unrelated BAC by the means

large fragment cloning-Molecular Biology

Hi I have a 9kb fragment already cloned into bluescript, and I am trying to insert an extra 3.5kb. I've been trying for some time now with no success - when I do get colonies very few have any of the new insert

DNA Recovery With Low Melt Agarose

Recovery of DNA from Low Melting Point Agarose Gels 1.Run digestion products on 0.7% LMP agarose gel in 1X TBE (it's nice to have at least 1ug of the fragment you want). LMP agarose is fragile; pour gel with EtBr 2.Let solidify in cold room