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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
上海恪敏生物科技有限公司
- 规格:
50preps
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文献和实验Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992) Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
of 5 M potassium acetate solution (pH 4.8) . This solution neutralizes NaOH in the previous lysis step while precipitating the genomic DNA and SDS in an insoluble white, rubbery precipitate. Spin at top speed 1 min. 6. Transfer supernatant to new
Simple Miniprep DNA Preparation
SNGE,NIMH,NIH http://intramural.nimh.nih.gov/lcmr/snge/Protocols/Miniprep.html SOLUTIONS: •Resuspension buffer: 25mM Tris-HCl (ph8) 50mM Na2 EDTA 1% glucose Autoclave and store at 4℃. •NaOH/SDS: 0.2 N NaOH/1% SDS. Prepare fresh
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