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- ZYMO RESEARCH
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- 2026年01月03日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Quick-DNA/RNA Miniprep
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
RT
HIGHLIGHTS
- Quick & Easy: Extract DNA and RNA from cells, small amounts of easy-to-lyse tissue, buffy coat, buccal cells, plasma, serum, and other biological liquids. Not compatible with whole blood.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
DESCRIPTION
The Quick-DNA/RNA Miniprep Kit provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue. The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol – no extra steps are required! Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes.
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
实验原理 If using the SQ DNA/RNA/Protein for the first time, please read this booklet to become familiar with the procedure and its various modifications. Samples are first lysed in a specially formulated buffer. The protein
with 1 volume of chloroform x1, spin 2 min. Transfer aqueous phase to a new tube. 9) Add 1 volume of isopropanol, mix. Spin at max. speed x 2 min. 10) Rinse with 70% ethanol. Dry pellet. Resuspend in 80 µL of T.E. Optional (for DNA sequencing) 11) Add 20
Miniprep/Kit-free high-throughput protocol
Background This protocol is adapted from "Molecular Cloning: A Laboratory Manual", Second Edition, Sambrook, Fritsch, and Maniatis. It is a quick, inexpensive way to purify large numbers of plasmids (I used to routinely do 80 at a time
