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- 文献和实验
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- 库存:
10
- 供应商:
上海恪敏生物科技有限公司
- 规格:
250 pack
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文献和实验Here we describe an ion exchange chromatography based method–-the use of anion or cation exchange (AEX or CEX) mini spin columns–-for sample fractionation. Using rat brain tissues, we demonstrate that these mini spin columns provide an easy, convenient, and reproducible
columns, and adsorption to a polyvinylidene difluoride (PVDF) membrane in the ProSorb sample preparation cartridge from Applied Biosystems. Both methods work well, with somewhat better recoveries for the gel filtration method.
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
Equilibration Buffer inro the column. Incubate at room temperature for 5 minutes. Spin at maximum speed (>13,000 x g) for 1 minute. 9. Apply 750 ul of mixture from step 7 to the HiBind® Mini column II (purple) pre-inserted in a 2 ml collection tube
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