Recombinant Human Phosphoinositide 3-kinase p110d/p85a
Synonyms Phosphoinositide 3-kinase p110d/p85a, PI3Kd.
Description The class IA PI3K? is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3K? in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3K? in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein’s application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110? chain is 120.2 kDa (without Tag) and the p85? chain 83.5 kDa.
Recombinant Human Phosphoinositide 3-kinase p110d/p85aSource Sf9 insect cells.
Physical Appearance Sterile filtered liquid formualtion.
Formulation PI3Kd solution in 25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol.
Recombinant Human Phosphoinositide 3-kinase p110d/p85aStability PI3Kd although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by SDS Page.
Activity 3,000 U/mg.
Recombinant Human Phosphoinositide 3-kinase p110d/p85aUnit Definition 1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.