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Recombinant Lysostaphin

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  • ¥1680 - 3988
  • 美国ATCC
  • 美国/中国
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 供应商

      钰博生物

    • 细胞类型

      复苏

    • 组织来源

      见说明书

    • 物种来源

      人、大鼠、小鼠、、、、

    • 免疫类型

      请咨询销售人员

    • 细胞形态

      上皮样;多角形

    • 是否是肿瘤细胞

      见说明书

    • 器官来源

      见说明书

    • 运输方式

      干冰运输or活细胞运输

    • 年限

      见说明书

    • 生长状态

      贴壁生长

    Recombinant Lysostaphin
    Synonyms Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase.
    Description Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.
    The Mw of lysostaphin is 26,921 (Recsei et al, PNAS 1987).
    Lysostaphin has optimal stability in the range of pH 4.5, and optimal activity in the range of pH 8.
    For a stock solution it is recommended to work with 10mg/ml lysostaphin in 10mM sodium acetate pH 4.5. For a reaction buffer it is recommended to work with 200mM Tris-HCl pH 8.
    Recombinant LysostaphinSource Escherichia Coli.
    Physical Appearance Sterile Filtered lyophilized powder.
    Formulation The protein was lyophilized without any additives.
    Stability Lysostaphin although stable at 4℃ for 6 months, should be stored desiccated below -18℃.
    Please prevent freeze-thaw cycles.
    Recombinant LysostaphinSolubility It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5, which can then be further diluted to other aqueous solutions.
    Purity 96.9% as determined by RP-HPLC.
    Biological Activity Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH8.0, 30℃.
    Specific Activity Determined to be 4,243 units/mg.
    Protein content Protein quantitation was carried out by two independent methods
    1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.
    Recombinant LysostaphinUsage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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