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- xy-521Mu01
- 2025年07月11日
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- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)
- 库存：
1000
- 供应商：
上海信裕
黏膜地址素细胞黏附分子(MAdCAM1)重组蛋白
Recombinant Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | xy521Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 30.4kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
黏膜地址素细胞黏附分子(MAdCAM1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- PE SEVAVAMGTS LQITCSMSCD EGVARVHWRG LDTSLGSVQT LPGSSILSVR GMTGTPV CVGSCGSRSF QHSVKILVYA FPDQLVVSPE FLVPGQDQVV SCTAHNIWPA DPNSLSFALL LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR MTQRWRLPSL GTPAPPALHC QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT SSSTAEAVST GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASC
MGHHHHHHSG SEF- PE SEVAVAMGTS LQITCSMSCD EGVARVHWRG LDTSLGSVQT LPGSSILSVR GMTGTPV CVGSCGSRSF QHSVKILVYA FPDQLVVSPE FLVPGQDQVV SCTAHNIWPA DPNSLSFALL LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR MTQRWRLPSL GTPAPPALHC QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT SSSTAEAVST GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASC
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
黏膜地址素细胞黏附分子(MAdCAM1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
黏膜地址素细胞黏附分子(MAdCAM1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
黏膜地址素细胞黏附分子(MAdCAM1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
程度的重叠与区别。迄今为止，外泌体的分离方法尚无公认的“金标准”。由于技术的限制，无论采用何种方法，都无法将外泌体与其他EV 亚群彻底分离，因此，目前在递送载体方面的研究和应用中，外泌体常与其他 EV 亚群混合使用。哺乳动物和植物细胞均能产生外泌体。外泌体是一个具有高度异质性的群体，其异质性表现在多个方面，包括大小异质性、包含物异质性、功能异质性和细胞来源异质性。不同细胞来源的外泌体其大小、表面受体和内容物往往具有不同特征。细胞靶向分子、细胞黏附分子、代谢物和核酸等在外泌体的形成过程中被包裹在囊泡腔内
的半透膜，富含毛细管，培养时纤维管内灌流充以氧气的无血清培养液，管外壁则供细胞黏附生长，营养物质通过半透膜从管内渗透出来供细胞生长；对于血清等大分子营养物，划必须从管外灌入，否则会被半透膜阻隔不能被细胞利用；细胞的代谢废物也可通过半透膜渗入管内，避免了过量代谢物对细胞的毒害作用。 优点是： 占地空间少； 细胞产量高，细胞密度可达109数量级； 生产成本低，且细胞培养维持时间长，适用于长期分泌的细胞。 六、生物反应器的设计和放大 设计的总体考虑是： ①结构严密，能耐受蒸汽
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黏膜地址素细胞黏附分子(MAdCAM1)重组蛋白
¥1 - 3980
