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文献和实验Sodium Acetate Precipitation of Small Nucleic Acids
实验步骤 1. Add 2 μl carrier to the nucleic acid solution and mix well. 2. Add 1:10 volume of 3 M sodium acetate and mix thoroughly; for 230 μl of Lower Running Buffer, this will be 23 μl of 3 M
Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
% ethanol Measure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final concentration of 0.3 M) or an equal volume of 5 M ammonium acetate (final concentration of 2.0-2.5 M
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