人神经传导与膜转运定量PCR芯片试剂盒

ScienCell's GeneQuery™ human neural transmission and membrane trafficking qPCR array kit (GQH-NTM) is designed to facilitate gene expression profiling of key genes involved in (i) human neurotransmission; (ii) membrance trafficking; and (iii) related diseases such as generalized anxiety disorder, DDC deficiency, TH deficiency, and SPR deficiency. 88 genes are selected in this kit based on public database and literature research.

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Neural Transmission and Membrane Trafficking qPCR Array Plate Layout* (8 controls in Bold and Italic)

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A

ADRA1A

BDNF

CHRNA5

DRD2

GABRA2

GABRG3

GRIK1

GRM3

HTR2A

OPTN

SNAP25

β-actin

B

ADRA1D

BTD

CHRNA6

DRD4

GABRA4

GABRQ

GRIK2

GRM4

HTR2C

OTC

SOD1

GAPDH

C

ADRA2A

CHMP2B

CHRNA7

DRD5

GABRA5

GABRR2

GRIK4

GRM6

HTR3A

PTS

SPR

LDHA

D

ADRB2

CHRM1

CHRNE

FIG4

GABRB1

GCH1

GRIK5

GRM7

HTR4

QDPR

TARDBP

NONO

E

ADRB3

CHRM4

COMT

FUS

GABRB3

GH1

GRIN2A

HTR1A

HTR7

RAB10

TH

PPIH

F

AKR1B1

CHRM5

DBH

G6PD

GABRE

GRIA1

GRIN2B

HTR1B

LLGL1

SLC6A2

TPH1

GDC

G

ALDH5A1

CHRNA3

DHFR

GABBR1

GABRG1

GRIA2

GRIN2C

HTR1D

LPL

SLC6A3

VAPB

PPC

H

ALS2

CHRNA4

DRD1

GABBR2

GABRG2

GRIA3

GRM1

HTR1F

MAOA

SLC6A4

VCP

NTC

* gene selection may be updated based on new research and development