ScienCell's GeneQuery™ human apoptosis qPCR array kit (GQH-APO) is designed to facilitate gene expression profiling of key genes involved in human apoptosis. 88 genes are selected in this kit based on public database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Apoptosis qPCR Array Plate Layout* (8 controls in Bold and Italic)
1
2
3
4
5
6
7
8
9
10
11
12
A
ABL1
BAG1
BIRC1
BRAF
CASP6
DFFA
IL1RAP
MYD88
PIK3R3
RELA
TNFRSF9
β-actin
B
AIFM1
BAG3
BIRC2
CAPN1
CASP9
DIABLO
IL3
NGF
PPP3CA
RIPK1
TNFSF10
GAPDH
C
AKT1
BCL10
BIRC3
CAPN2
CD40
ENDOG
IRAK2
NOD1
PPP3CB
RIPK2
TP53
LDHA
D
AKT2
BCL2
BIRC5
CASP1
CFLAR
FAS
IRAK3
NOL3
PPP3R1
TNF
TP53BP2
NONO
E
AKT3
BCL2L1
BIRC6
CASP10
CHUK
HRK
IRAK4
PIK3CB
PRKAR1A
TNFRSF10B
TRADD
PPIH
F
APAF1
BCL2L10
BNIP2
CASP2
CIDEA
IGF1R
LTBR
PIK3CG
PRKAR2B
TNFRSF10D
TRAF2
GDC
G
ATM
BID
BNIP3
CASP3
CRADD
IL1B
MAP3K14
PIK3R1
PRKX
TNFRSF11B
TRAF3
PPC
H
BAD
BIK
BNIP3L
CASP4
DAPK1
IL1R1
MCL1
PIK3R2
PYCARD
TNFRSF1B
XIAP
NTC
* gene selection may be updated based on new research and development