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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Matrix Metalloproteinase 13 (MMP13)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Oryctolagus cuniculus (Rabbit) |
| Product No. | xy099Rb02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 20.3kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGHHHHHHSGS- SLSL DAITSLRGET MIFKDRFFWR LHPQQVDAEL FLTKSFWPEL PNRIDAAYEH PARDLIFIFR GKKFWAPNGY DILEGYPQKL SELGFPREVK KISAAVHFED TGKTLFFSGN QVWSYDDTNH TMDQDYPRLI EEEFPGIGGK VDAVYEKNGY IYFFNGPIQ
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
基质金属蛋白酶13(MMP13)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验M/NM_000632 calmodulin 2/NM_001743 nuclear mitotic apparatus protein 1/NM_006185 oligodendrocyte lineage transcription factor 2/NM_005806 Mmp13 Mmp1 [url=]http://www.dxy.cn/bbs/thread/1315321CEA [/url] GAPDH cyclin-dependent kinase inhibitor
2008 Publisher: Springer-Verlag New York, LLC Series: Nucleic Acids and Molecular Biology Series , #22 ISBN-13: 9783540709374 ISBN: 3540709371
),最终结果还是部分蛋白未溶解,导致蛋白活性的减弱。 3) 一定不能振荡(vortex)。 这里所说的振荡是指用涡旋仪进行快速振荡。 有用户会问,若想保证溶解液与冻干粉充分混匀,以实现细胞因子或重组蛋白的完全溶解,该怎么办呢? 答:多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般我们用移液枪的枪头轻吹几下,即可使细胞因子或重组蛋白完全溶解。 对于不易溶解的细胞因子或蛋白,PeproTech会在说明书中进行备注(Note)。如在重组人IL-13(产品
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