相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Heme Oxygenase 1, Decycling (HO1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Sus scrofa; Porcine (Pig) |
| Product No. | xy584Po01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 18.8kDa |
| Concentration | 5.8 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- LEEEIEHNKE NPVYTPLYFP EELHRRAALE QDMAFWYGPR WQEAIPYTQA TKRYVRRLQQ VGRFEPELLV AHAYTRYMGD LSGGQVLKKI AQKALDLPSS GEGLAFFTFP NV
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
血红素氧合酶1(HO1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验生成素(EPO)转铁蛋白、血红素加氧酶-1等的基因,它们都有一个共同的顺式作用元件(CGTG),有利于在5’或3’侧翼区的低氧诱导低氧诱导作用因子-1(HIF-1)和低氧反应增强子(HRE)结合,激活靶基因的转录,在低氧浓度下可使重组蛋白大量表达。据报道红细胞生成素启动子在1%氧浓度比在21%氧浓度下活陛增强100倍。这又是一种新的提高表达量的作用机制。 4.提高蛋白表达产量的措施 哺乳动物细胞对培养环境十分敏感,营养和生长因子缺乏、缺氧、病毒感染、毒性代谢
。这些启动子在诱导前后活性可相差4个数量级。另外,在哺乳动物细胞中已发现存在大量在低氧环境中可诱导转录的基因,如编码红细胞生成素(EPO)转铁蛋 白、血红素加氧酶-1等的基因,它们都有一个共同的顺式作用元件(CGTG ),有利于在5’或3’侧翼区的低氧诱导低氧诱导作用因子-1(HIF-1)和低氧反应增强子(HRE)结合,激活靶基因的转录,在低氧浓度下可使重组蛋白大量表达。据报道红细胞生成素启动子在 1%氧浓度比在21%氧浓度下活陛增强100倍。这又是一种新的提高表达量的作用机制。 2、宿主
在BVR过表达的293A细胞中,研究BVR促进转录因子2(ATF2)和血红素加氧酶1(HO1)升高的机制,前者是信号通路基因。研究认为BVR可能通过两个作用位点(调控元件),即AP-1位点和CRE位点来调控基因的转录。 在研究中,对BVR过表达的293A细胞用Superarray的信号通路发现者基因芯片(GEArray Q Series Human Signal Transduction PathwayFinder Gene Array, Catalog No. HS-008N)进行了检测,该芯片
技术资料暂无技术资料 索取技术资料




