Recombinant Mouse Interleukin-

Synonyms IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.

Introduction Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.

Recombinant Mouse Interleukin-6Description Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.

The IL-6 is purified by proprietary chromatographic techniques.

Source Escherichia Coli.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Recombinant Mouse Interleukin-6Formulation The protein was lyophilized without any additives.

Solubility It is recommended to reconstitute the lyophilized Interleukin 6 in 10mM HCl to 0.1-1.0 mg/ml to regain full activity, and can then be further diluted to other aqueous solutions.

Stability Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity Greater than 95.0% as determined by SDS-PAGE.

Recombinant Mouse Interleukin-6Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.

Biological Activity The ED50 as determined by the dose-dependant induction of 7TD1 cell proliferation, is 0.018 ng/ml corresponding to a Specific Activity of 55MIU/mg.

References Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.

Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .

Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.