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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Mus musculus (Mouse) |
| Product No. | xy219Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 19.8kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
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Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
肽基脯氨酰顺反式异构酶NIMA相互作用蛋白1(PIN1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验【求助】P53的翻译后修饰都有哪些? 怎样验证其修饰后与靶标启动子的结合活性的变化?
、Ser376)则和p53降解的增加有关。 顺反异构化修饰 p53的活化涉及构象的改变,由肽脯氨酰-顺反异构酶︱(PIN1)引起的特定的脯氨酸残基的顺反异构化可导致构象的改变。PIN1的蛋白结合位点包括一个磷酸化的丝氨酸或苏氨酸及其后紧跟的一个脯氨酸,从而催化脯氨酸残基发生顺反异构化。目前对Ser127-Pro128、Thr150-Pro151基序是否受PIN1的靶向作用还不清楚。PIN1引起的p53构象的改变阻止了MDM2的结合和/或促进了MDM2的解离,从而使
, DnaK 功能:参与蛋白质折叠,转运和装配。 (2) 折叠催化剂 A、 蛋白质二硫键异构酶(Protein disulfide isomerases, PDI) Dsb家族 功能:进行巯基-二硫键交换,参与二硫键形成和重排。 B、肽基脯氨酸异构酶(Peptidyl-prolyl isomerases, PPI) RotA, FkpA, SurA和Skp/OmpH 功能:对Xaa-Pro肽键进行异构化,缓慢地对构型进行重排。 七、存在问题 1. 表达产物翻译后不能有效地修饰和加工
集团组成,故不属于去垢剂,不会形成微束,易于透析去除,目前,常用的有NDSB-195,NDSB-201,NDSB-256。[8] 2.2.2.3PEG-NaSO4两相法 用PEG和NaSO4作为成相剂,然后加入盐酸胍,再把变性的还原的蛋白质溶液加入其中进行复性,但这种方法需复性的变性蛋白质的浓度必须低。[9] 2.2.2.4分子伴侣和折叠酶等 这类蛋白质主要包括硫氧还蛋白二硫键异构酶、肽酰-辅氨酰顺反异构酶、分子伴侣、FK506结合蛋白、Cyclophilin等。分子伴侣和折叠酶
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